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Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an <t>oCelloscope</t> TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.
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Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an <t>oCelloscope</t> TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.
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Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an <t>oCelloscope</t> TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.
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Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an <t>oCelloscope</t> TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.
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Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an oCelloscope TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.

Journal: bioRxiv

Article Title: Improving the production and virulence of entomopathogenic fungi for biological control using insect-derived in vitro culture medium

doi: 10.64898/2026.03.14.711814

Figure Lengend Snippet: Four isolates of each species were used and grown on three locust-derived media (A – C) and the ¼ SDAY control medium (D). Medium A represents the twice filtered locust medium, Medium B represents the once filtered locust medium, and Medium C represents the unfiltered locust medium. Over the course of 7 days, radial growth and sporulation were monitored using a Reshape imaging robot. Thereafter, conidia were harvested to measure spore count, germination rate, and maximum growth in ¼ SDY medium using an oCelloscope TM imaging system. Lastly, pathogenicity assays were conducted using selected M. brunneum isolates on using Tenebrio molitor larvae.

Article Snippet: Each isolate x medium of origin combination was plated in triplicate and the oCelloScope Imaging system (BioSense Solutions, Denmark) was used to track the germination and growth of the isolates over the course of 48 hours.

Techniques: Derivative Assay, Control, Imaging